Journal: Biology Open

Article Title: Divergent Hemogen genes of teleosts and mammals share conserved roles in erythropoiesis: analysis using transgenic and mutant zebrafish

doi: 10.1242/bio.035576

Figure Lengend Snippet: Promoter elements have distinct roles in driving hematopoietic, renal and testicular expression of Hemogen in transgenic Tg(Hemgn:mCherry) zebrafish. (A) Schematic of the zebrafish Hemogen gene. CNEs, black; coding exons, white; transcription initiation sites, bent arrows. Three Tg(Hemgn:mCherry,myl7:EGFP) transgenes driven by portions of the Hemogen promoter were transfected into one-cell TU embryos by Tol2 transposase-mediated insertion. Numbers indicate length of promoter elements and arrows show gene direction. (B) 20 hpf. P1 transgene expression in the peripheral blood island (PBI). (C) 72 hpf. P1 transgene expression in the pronephric ducts (PD). (D) 5 dpf. P1 transgene expression in the proximal convoluted tubule (PCT). (E,F) 72 hpf. Co-localization of mCherry and EGFP in progenitors in the CHT of Tg(Hemgn-P1:mCherry,Lcr:GFP) or Tg(Hemgn-P1:mCherry,CD41:EGFP) zebrafish. (G) Transgene expression in mature erythrocytes from adult zebrafish. (H) Transgene expression in adult head kidney (HK), trunk kidney (TK) and tail kidney (T) near the EGFP+ heart (H). (I) Transgene expression in adult Sertoli cells (Se) that surround the seminiferous tubules (ST). (J) Proportion of embryos expressing transgenes P1, P2 or P3 in ICM, kidney, CHT and circulating primitive erythrocytes (RBC). Scale bars: 100 µm (B,D-F,I); 500 µm (C,H); 25 µm (G).

Article Snippet: All transgenic lines, with the exception of nuz3 , are available through the Zebrafish International Resource Center.

Techniques: Expressing, Transgenic Assay, Transfection

Journal: Development (Cambridge, England)

Article Title: A key role for foxQ2 in anterior head and central brain patterning in insects

doi: 10.1242/dev.147637

Figure Lengend Snippet: Loss of Tc-foxQ2 function leads to brain defects. (A-C) Glial tissue (black) is visualized in L1 larvae by the transgenic brainy reporter line in WT (A) and Tc-foxQ2 RNAi (B,C). Voltex projections. (D-H) Mushroom bodies (MBs; black) are visualized by the transgenic MB-green reporter line in WT (D,D′) and Tc-foxQ2 RNAi (E-H). MAX projections; D′, 3D projection. (A) WT L1 larval brain showing the two brain hemispheres with MBs (magenta), antennal lobes (cyan), and the midline-spanning central body (CB; yellow). (B) A weak Tc-foxQ2 RNAi larval brain phenotype showing loss of the boundary between the medial lobes of the MB (compare open arrowheads in B and A). The CB appears to be reduced in size. (C) Intermediate phenotype Tc-foxQ2 RNAi larval brains appear to lack the MBs and the CB appears reduced in size. (B,C) In both phenotypes the brain hemispheres appear to be fused (solid arrowhead). (D) WT L1 larval MBs in dorsal view. (D′) 3D projection of WT L1 larval MBs, providing an overview of the organization of the structures. (E) A weak Tc-foxQ2 RNAi-induced MB phenotype, where the two medial lobes appear to be fused (compare arrowheads in D and E). (F) MBs with distorted pedunculi, leading to a loss of contact between the two medial lobes (arrowheads), and reduced vertical lobes (arrow). (G) Phenotype with interdigitating MBs. (H) In strong phenotypes the MB structures are highly reduced or absent. OL, optical lobe; mL, medial lobe; Pe, pedunculus; vL, vertical lobe; Ca, calyx.

Article Snippet: Voltex projections. (D-H) Mushroom bodies (MBs; black) are visualized by the transgenic MB-green reporter line in WT (D,D′) and Tc-foxQ2 RNAi (E-H).

Techniques: Transgenic Assay

Journal: Current biology : CB

Article Title: The Drosophila pioneer factor Zelda modulates the nuclear microenvironment of a Dorsal target enhancer to potentiate transcriptional output

doi: 10.1016/j.cub.2019.03.019

Figure Lengend Snippet: (A-B) Conventional enzymatic in situ hybridization staining of sog in wild type and zld mutant NC14 embryos. (C) Schematic representation of transgenes. MS2 loops have been incorporated into the 5’ end of the transcript upstream of a lacZ reporter sequence. (D-E) in situ hybridization staining of the engineered MS2v5(-TAG) lacZ transgenic embryos, showing that 3TAG and 0TAG expression is similar to the expression of sog in wild type and zld mutants, respectively.

Article Snippet: Transgenic lines carrying these constructs were generated by phiC31 integration in the 53B2 landing site (VK00018), Bloomington stock number 9736 [ 32 , 33 ] by BestGene.

Techniques: In Situ Hybridization, Staining, Mutagenesis, Sequencing, Transgenic Assay, Expressing